Abstract To assess the potential impact of predominant circulating SARS-CoV-2 variants on neutralizing activity of infected and/or vaccinated individuals, we analyzed neutralization of pseudoviruses expressing the spike of the original Wuhan strain, the D614G and B.1.1.7 variants. Our data show that parameters of natural infection (time from infection and infecting variant) determined cross-neutralization. Importantly, upon vaccination, previously infected individuals developed equivalent B.1.1.7 and Wuhan neutralizing responses. In contrast, uninfected vaccinees showed reduced neutralization against B.1.1.7. Funding This study was funded by Grifols, the Departament de Salut of the Generalitat de Catalunya , the Spanish Health Institute Carlos III, CERCA Programme/ Generalitat de Catalunya , and the crowdfunding initiatives #joemcorono, BonPreu/Esclat and Correos.

Previous SARS-CoV-2 infection increases B.1.1.7 cross-neutralization by vaccinated individuals