Abstract Microsatellites density landscapes of all 24 chromosomes were built here for the first complete reference sequence of human genome T2T-CHM13. These landscapes showed that short tandem repeats (STRs) are prone to aggregate characteristically to form a large number of STRs density peaks. We classified 8,823 high microsatellites density peaks (HMDPs), 35,257 middle microsatellites density peaks (MMDPs) and 199, 649 low microsatellites density peaks (LMDPs) on the 24 chromosomes; and also classified the motif types of every microsatellites density peak. And 514 genomic regions were characterized by microsatellite density feature in the full T2T-CHM13 genome. The landscape maps revealed that the local microsatellites density varies enormously and rhythmically along the every chromosome of T2T-CHM13.

Microsatellite Density Landscapes Illustrate Short Tandem Repeats Aggregation in The Complete Reference Human Genome