Abstract The accelerating environmental crisis has intensified the demand for switching from traditional economy to a renewable one with a reduced carbon footprint. Here we reported a hybrid system, coupling chemical process of CO 2 hydrogen reduction and biological process for polyhydroxybutyrate (PHB) synthesis, that utilized CO 2 as a raw material to produce PHB in vitro. The synthetic pathway of PHB was optimized by screening more efficient methanol oxidases, high activity mutants of glycolaldehyde synthase and coordinating enzyme dosages in the pathway, which achieved the carbon yield of 93.6% for producing PHB from methanol. Finally, by combining with the chemical process from CO 2 to methanol, a scaling-up bio-system was performed to convert CO 2 into PHB, yielding 5.8 g/L with the productivity of 1.06 g -1 L -1 h -1 . This approach represents a promising carbon-neutral way to produce biodegradable plastics.

Hybrid Synthesis of bioplastics polyhydroxybutyrate from carbon dioxide