Abstract Monocular deprivation (MD) causes an initial decrease in synaptic responses to the deprived eye in juvenile mouse primary visual cortex (V1) through Hebbian long-term depression (LTD). This is followed by a homeostatic increase, which has been attributed to synaptic scaling. However, homeostasis during other forms of visual deprivation is caused by sliding the threshold for Hebbian long-term potentiation (LTP) rather than scaling. We therefore asked whether the homeostatic increase during MD requires GluN2B-containing NMDA receptor activity, which is required to slide the plasticity threshold but not for synaptic scaling. Selective GluN2B blockade from 2-6d after monocular lid suture prevented the homeostatic increase in miniature excitatory postsynaptic current (mEPSC) amplitude in monocular V1 of acute slices and prevented the increase in visually evoked responses in binocular V1 in vivo . The decrease in mEPSC amplitude and visually evoked responses during the first 2d of MD also required GluN2B activity. Together, these results indicate that GluN2B-containing NMDA receptors first play a role in LTD immediately following eye closure, and then promote homeostasis during prolonged MD by sliding the plasticity threshold in favor of LTP.

GluN2B-containing NMDA receptors are required for potentiation and depression of responses in ocular dominance plasticity