Abstract Variability in the chemical composition of human scent has the potential to modulate mosquito attraction to certain humans. We have engineered a large-scale, semi-field system in Zambia for quantifying mosquito olfactory preferences towards whole body odor sourced from different humans under naturalistic conditions. In a flight cage arena with infrared tracking, we document that the African malaria mosquito Anopheles gambiae hierarchically prefers to land on heated targets mimicking human skin temperature when they are baited with carbon dioxide (CO 2 ) over background air, human body odor over CO 2 , and the scent of one individual over another. In a six-choice assay configuration, we further identify humans at both ends of the attractiveness spectrum whose scent is differentially attractive to An. gambiae relative to other individuals. We demonstrate integrative use of this multi-choice olfactory assay with whole body volatilomics, establishing a powerful method for discovery of human odorants modulating heterogeneity in biting risk at enhanced throughput.

A semi-field system for quantifyingAnopheles gambiaeattraction to human scent