ABSTRACT Microbial genomes produced by single-cell amplification are largely incomplete. Here, we show that primary template amplification (PTA), a novel single-cell amplification technique, generated nearly complete genomes from three bacterial isolate species. Furthermore, taxonomically diverse genomes recovered from aquatic and soil microbiomes using PTA had a median completeness of 81%, whereas genomes from standard amplification approaches were usually &lt;30% complete. PTA-derived genomes also included more associated viruses and biosynthetic gene clusters.

scMicrobe PTA: Near Complete Genomes from Single Bacterial Cells