Abstract

While impairing neuronal metabolism limits brain performance, it remains poorly understood whether enhancing metabolism in neurons, in contrast, could boost brain function. We find that reducing the expression of the mitochondrial H+/Ca2+ exchanger Letm1 results in increased Ca2+ retention in the mitochondrial matrix of firing neurons, which overactivates neuronal metabolism in flies and rodents. We find that upscaled metabolic states in active neurons of central memory circuits of flies and mice enable storing long-term memories in training paradigms in which wild-type counterparts of both species fail to remember. Our findings unveil an evolutionarily conserved mechanism that controls mitochondrial metabolism in active neurons and prove its crucial role in governing higher brain functions, such as long-term memory formation. Highlights- Letm1 controls activity-driven mitochondrial Ca2+ efflux in neurons - Increased mitochondrial Ca2+ retention during activity overactivates neuronal metabolism - Activity-driven upscaling of neuronal metabolism facilitates long-term olfactory memory in flies and mice