Abstract

Human amniotic fluid stem cell (hAFSC) exhibit significantly as a new treatment modality in hair loss, wound, scar and regenerative plastic surgery/dermatology. The difference of our study from previous amniotic fluid studies on the hair growth model was studied through the direct effect of human amniotic fluid (hAF). In our study, amniotic fluid was made acellular, pooled and freezed, making it more standardized in terms of its contents. Therefore, the present study is aimed at investigating the efficacy and safety of amniotic fluid in hair loss model on rat. Using the hair loss model rats, we investigated the therapeutic potential of freezing amniotic fluid (FAF) and freezing gama irradiated amniotic fluid (FAFI). Our results showed that FAF and especially FAFI increased number of total hair follicles and accelerated number of transition to anagenic hair follicle. We observed that increased rate of arginase+1(Arg+1)/CD68 activity around the hair follicles treated with hAF. Our study suggests that FAFI may represent a safe and effective tool for increasing hair follicle and accelerating to anagen stage during hair loss model.