Abstract

BackgroundCirculating HBV RNAs have been proposed as a biomarker that reflects the transcriptional activity of cccDNA and may help to evaluate HBV treatments activity. Different research assays have been proposed and, although 2 PCR-based research use only (RUO) investigational assays (IA) have been developed, the lack of standardized protocols represents an important limitation. Here we have designed and generated a stable clonal cell line producing an RNA-based standard for the calibration of PCR-based circulating HBV RNA assays.MethodsHBV RNA producing Huh7-derived stable cell lines were generated by transfecting pTriEX plasmids containing 1.1 length HBV DNA genomes carrying mutations in the catalytic site (YMAA mutation) and the TP-domain (Y63F) of the polymerase, and the ε-loop of the pgRNA (mutation A1G).ResultsThe clonal cell line (Huh7-3D29), carrying a double YMAA and Y63F mutation, displayed, and maintained over several passages in culture, a high RNA secretion phenotype with negligible residual secreted HBV DNA. Density gradient centrifugation showed that most of the secreted HBV RNA from Huh7-3D29 cells was detected in naked capsid and virions-like particles and only a minority in small extracellular vescicles (sEV). Nanopore sequencing of 5'RACE products shows that the majority of the Huh7-3D29 secreted HBV RNAs start at the 5' end of pgRNA and pgRNA derived spliced RNAs. Finally, Huh7-3D29 cells showed a high and up-scalable secreted RNA yield allowing 1,300 standard curves in 9 days from 1 flask.ConclusionWe generated a clonal cell line that produces high amounts of HBV RNAs with very low quantities of contaminating HBV DNAs, representing a stable source of RNA standard for HBV RNA assays calibration.Impact and ImplicationsSeveral investigational assays and 2 research use only (RUO) assays have been developed to detect and quantify circulating HBV RNAs, an emerging biomarker of cccDNA transcriptional activity and target engagement by new HBV treatments. The lack of a unique molecular standard for circulating HBV RNAs quantification represents an important limitation. Here we describe the generation of a stable clonal cell line producing and secreting an RNA-based standard containing all the HBV RNA species found in HBV patients' sera (e.g., pgRNA, HBx transcripts). This new RNA standard will serve for the calibration of all PCR-based assays for circulating HBV RNA quantification to evaluate in a non-invasive manner the size of the transcriptionally active cccDNA pool and the activity of novel strategies aiming at curing HBV infection.