Abstract

Digoxin (DGN), a widely used cardiac glycoside steroid for treating heart diseases. The prescription dosage range of digoxin is 0.5 – 2.0 ng/mL and concentration above 2 ng/mL can lead to intoxication. To monitor and detect digoxin in blood level is essential to minimize the endanger toxicity of human health. Addressing this concern, here we prepare a cerium manganese oxide (CMO) catalyst by a simple chemical coprecipitation method followed by heat treatment. The CMO morphological and structural characteristics were investigated by various spectroscopic techniques. The electrochemical properties of CMO catalyst were evaluated using cyclic voltammetry (CV) and differential pulse voltammetry (DPV) techniques, which also determined its electrochemical performance. Due to the high conductivity and large surface area with abundant active sites, CMO catalyst was used as a modified electrode material for detecting DGN in biological samples. The CMO sensor exhibits excellent electrocatalytic performance, with a dynamic linear range of 7.81 ×10–6 pg/L to 430 ×10–6 pg/L. The limit of detection and limit of quantification (LOD and LOQ) were found to be 1.99 ×10–6 pg/L and 6.05 ×10–6 pg/L, respectively. Furthermore, the sensor's efficiency was validated through selectivity test and analysis of real biological samples such as human blood and urine.