Simultaneously Enhanced Catalytic Activity and Thermostability of a Baeyer–Villiger Monooxygenase from Oceanicola granulosus by Reshaping the Binding Pocket

Abstract

Enzymatic oxygenation of various cyclic ketones into lactones via Baeyer-Villiger monooxygenases (BVMOs) could provide a promising route for synthesizing fragrances and pharmaceutical ingredients. However, unsatisfactory catalytic activity and thermostability restricted their applications in the pharmaceutical and food industries. In this study, we successfully improved the catalytic activity and thermostability of a Baeyer-Villiger monooxygenase (