Abstract

Natural compounds capable of increasing root depth and branching are desirable tools for enhancing stress tolerance in crops. We devised a sensitized screen to identify natural metabolites capable of regulating root traits in Arabidopsis. {beta}-cyclocitral, an endogenous root compound, was found to promote cell divisions in root meristems and stimulate lateral root branching. {beta}-cyclocitral rescued meristematic cell divisions in ccd1ccd4 biosynthesis mutants and {beta}-cyclocitral-driven root growth was found to be independent of auxin, brassinosteroid, and ROS signaling pathways. {beta}-cyclocitral had a conserved effect on root growth in tomato and rice and generated significantly more compact crown root systems in rice. Moreover, {beta}-cyclocitral treatment enhanced plant vigor in rice plants exposed to salt-contaminated soil. These results indicate that {beta}-cyclocitral is a broadly effective root growth promoter in both monocots and eudicots and could be a valuable tool to enhance crop vigor under environmental stress.\n\nOne Sentence Summary{beta}-cyclocitral is a metabolite of {beta}-carotene that was identified using a sensitized chemical screen and acts broadly across plants to enhance root growth and branching.