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Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition)
Authors
Andrea Cossarizza,Hyun‐Dong Chang
Andreas Radbruch,Andreas Acs,Dieter Adam,Sabine Adam‐Klages,William Agace,Nima Aghaeepour,Mübeccel Akdiş,Matthieu Allez,Larissa Almeida,Giorgia Alvisi,Graham Anderson,Immanuel Andrä,Francesco Annunziato,Achille Anselmo,Petra Bächer,Cosima Baldari,Sudipto Bari,Vincenzo Barnaba,Joana Barros‐Martins,Luca Battistini,Wolfgang Bauer,Sabine Baumgart,Nicole Baumgarth,Dirk Baumjohann,Bianka Baying,Mary Bebawy,Burkhard Becher,Wolfgang Beisker,Vladimı́r Beneš,Rudi Beyaert,Alfonso Blanco,Dominic Boardman,Christian Bogdan,Jessica Borger,Giovanna Borsellino,Philip Boulais,Jolene Bradford,Dirk Brenner,Ryan Brinkman,Anna Brooks,Dirk Busch,Martin Büscher,Timothy Bushnell,Federica Calzetti,Garth Cameron,Ilenia Cammarata,Xuetao Cao,Susanna Cardell,Stefano Casola,Marco Cassatella,Andrea Cavani,Antonio Celada,Lucienne Chatenoud,Pratip Chattopadhyay,Sue Chow,Eleni Christakou,Luka Čičin‐Šain,Mario Clerici,Federico Colombo,Laura Cook,Anne Cooke,Andrea Cooper,Alexandra Corbett,Antonio Cosma,Lorenzo Cosmi,Pierre Coulie,Ana Cumano,Ljiljana Cvetkovic,Van Dang,Chantip Dang‐Heine,Martin Davey,Derek Davies,Sara Biasi,Genny Zotto,Gelo Cruz,Michael Delacher,Silvia Bella,Paolo Dellabona,Günnur Deniz,Mark Dessing,James Paparello,Andreas Diefenbach,Francesco Dieli,Andreas Dolf,Thomas Dörner,Regine Dress,Diana Dudziak,Michael Dustin,Charles‐Antoine Dutertre,Friederike Ebner,Sidonia Eckle,Matthias Edinger,Pascale Eede,Götz Ehrhardt,Marcus Eich,Pablo Engel,Britta Engelhardt,Anna Erdei,Charlotte Esser,Bart Everts,Maximilien Evrard,Christine Falk,Todd Fehniger,Mar Felipo‐Benavent,Helen Ferry,Markus Feuerer,Andrew Filby,Kata Filkor,Simon Fillatreau,Marie Follo,Irmgard Förster,John Foster,Gemma Foulds,Britta Frehse,Paul Frenette,Stefan Frischbutter,Wolfgang Fritzsche,David Galbraith,Anastasia Gangaev,Natalio Garbi,Brice Gaudillière,Ricardo Gazzinelli,Jens Geginat,Wilhelm Gerner,Nicholas Gherardin,Kamran Ghoreschi,Lara Gibellini,Florent Ginhoux,Keisuke Goda,Dale Godfrey,Christoph Goettlinger,José González‐Navajas,Carl Goodyear,Andrea Gori,Jane Grogan,Daryl Grummitt,Andreas Grützkau,Claudia Haftmann,Jonas Hahn,Hamida Hammad,Günter Hämmerling,Leo Hansmann,Göran Hansson,Christopher Harpur,Susanne Hartmann,Andrea Hauser,Anja Hauser,David Haviland,David Hedley,Daniela Hernandez,Guadalupe Herrera,Martin Herrmann,Christoph Hess,Thomas Höfer,Petra Hoffmann,Kristin Hogquist,Tristan Holland,Thomas Höllt,Rikard Holmdahl,Pleun Hombrink,Jessica Houston,Bimba Hoyer,Bo Huang,Fang‐Ping Huang,Johanna Huber,Jochen Huehn,Michael Hundemer,Christopher Hunter,William Hwang,Anna Iannone,Florian Ingelfinger,Sabine Ivison,Hans‐Martin Jäck,Péter Jani,Beatriz Jávega,Stipan Jonjić,Toralf Kaiser,Tomáš Kalina,Thomas Kamradt,Stefan Kaufmann,Baerbel Keller,Steven Ketelaars,Ahad Khalilnezhad,Srijit Khan,Jan Kisielow,Paul Klenerman,Jasmin Knopf,Hui-Fern Koay,Katja Kobow,Jay Kolls,Wan Kong,Manfred Köpf,Thomas Korn,Katharina Kriegsmann,Hendy Kristyanto,Thomas Kroneis,Andreas Krueger,Jenny Kühne,Christian Kukat,Désirée Kunkel,Heike Kunze‐Schumacher,Tomohiro Kurosaki,Christian Kurts,Pia Kvistborg,Immanuel Kwok,Jonathan Landry,Olivier Lantz,Paola Lanuti,Francesca LaRosa,Agnés Lehuen,Salomé LeibundGut‐Landmann,Holden Maecker,Leslie Leung,Megan Levings,Andreia Lino,Francesco Liotta,Virginia Litwin,Yanling Liu,Hans‐Gustaf Ljunggren,Michael Lohoff,Giovanna Lombardi,Lilly Lopez,Miguel López‐Botet,Amy Lovett‐Racke,Erik Lubberts,Hervé Luche,Burkhard Ludewig,Enrico Lugli,Sebastian Lunemann,Laura Maggi,Orla Maguire,Florian Mair,Kerstin Mair,Alberto Mantovani,Rudolf Manz,Aaron Marshall,Alicia Martínez‐Romero,Glòria Martrus,Ivana Marventano,Włodzimierz Maśliński,Giuseppe Matarese,Anna Mattioli,Christian Maueröder,Alessio Mazzoni,James McCluskey,Mairi McGrath,Helen McGuire,Iain McInnes,Henrik Mei,Fritz Melchers,Dror Fixler,Dirk Mielenz,Stephen Miller,Kingston Mills,Hans Minderman,Jenny Mjösberg,Jonni Moore,Barry Moran,Lorenzo Moretta,Tim Mosmann,Susann Müller,Gabriele Multhoff,Luis Muñoz,Christian Münz,Toshinori Nakayama,Milena Nasi,Katrin Neumann,Lai Ng,Antonia Niedobitek,Sussan Nourshargh,Gabriel Núñez,José‐Enrique O’Connor,Aaron Ochel,Anna Oja,Diana Ordonez,Alberto Órfão,Eva Orlowski‐Oliver,Wenjun Ouyang,Annette Oxenius,Raghavendra Palankar,Mir‐Farzin Mashreghi,Kovit Pattanapanyasat,Malte Paulsen,Dinko Pavlinić,Livius Penter,Pärt Peterson,Christian Peth,Jordi Pétriz,Federica Piancone,Winfried Pickl,Silvia Piconese,Marcello Pinti,A. Pockley,Malgorzata Podolska,Zhiyong Poon,Katharina Pracht,Immo Prinz,Carlo Pucillo,Sally Quataert,Linda Quatrini,Kylie Quinn,Helena Radbruch,Tim Radstake,Susann Rahmig,Hans‐Peter Rahn,Bartek Rajwa,Gevitha Ravichandran,Yotam Raz,Jonathan Rebhahn,Diether Recktenwald,Dorothea Reimer,Caetano Sousa,Ester Remmerswaal,Lisa Richter,Laura Rico,Andrew Riddell,Aja Rieger,J. Robinson,Chiara Romagnani,Anna Rubartelli,Jürgen Ruland,Armin Saalmüller,Yvan Saeys,Takashi Saito,Shimon Sakaguchi,Francisco Sala‐de‐Oyanguren,Yvonne Samstag,Sharon Sanderson,Inga Sandrock,Angela Santoni,R. Sanz,Marina Saresella,Catherine Sautès‐Fridman,Birgit Sawitzki,Linda Schadt,Alexander Scheffold,Hans Scherer,Matthias Schiemann,Frank Schildberg,Esther Schimisky,Andreas Schlitzer,Josephine Schlosser,Stephan Schmid,Steffen Schmitt,Kilian Schober,Daniel Schraivogel,Wolfgang Schuh,Thomas Schüler,Reiner Schulte,Axel Schulz,Sebastian Schulz,Cristiano Scottà,Daniel Scott‐Algara,David Sester,T. Shankey,Bruno Silva‐Santos,James Shelhamer,Katarzyna Sitnik,Silvano Sozzani,Daniel Speiser,Josef Špidlen,Anders Ståhlberg,Alan Stall,Natalie Stanley,Regina Stark,Christina Stehle,Tobit Steinmetz,Hannes Stockinger,Yousuke Takahama,Kiyoshi Takeda,Leonard Tan,Attila Tárnok,Gisa Tiegs,Gergely Toldi,Julia Tornack,Elisabetta Traggiai,Mohamed Trebak,Timothy Tree,Joe Trotter,John Trowsdale,Maria Tsoumakidou,Henning Ulrich,Sophia Urbanczyk,Willem Veen,Maries Broek,Edwin Pol,Sofie Gassen,Gert Isterdael,René Lier,Neil Hunter,Salvador Vento‐Asturias,Paulo Vieira,David Voehringer,Hans‐Dieter Volk,Anouk Borstel,K. Volkmann,Ari Waisman,Rachael Walker,Paul Wallace,Sa Wang,Xin Wang,Michael Ward,Kirsten Ward‐Hartstonge,Klaus Warnatz,Gary Warnes,Sarah Warth,Claudia Waskow,James Watson,Carsten Watzl,Leonie Wegener,Thomas Weisenburger,Annika Wiedemann,Jürgen Wienands,Anneke Wilharm,Robert Wilkinson,Gerald Willimsky,James Wing,Rieke Winkelmann,Thomas Winkler,Oliver Wirz,Alicia Wong,Peter Wurst,Jennie Yang,Juhao Yang,Maria Yazdanbakhsh,Liping Yu,Alice Yue,Hanlin Zhang,Yi Zhao,Susanne Ziegler,Christina Zielinski,Jakob Zimmermann,Arturo Zychlinsky
+433 authors
,Charles–Antoine DutertrePublished
Oct 1, 2019
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Abstract
These guidelines are a consensus work of a considerable number of members of the immunology and flow cytometry community. They provide the theory and key practical aspects of flow cytometry enabling immunologists to avoid the common errors that often undermine immunological data. Notably, there are comprehensive sections of all major immune cell types with helpful Tables detailing phenotypes in murine and human cells. The latest flow cytometry techniques and applications are also described, featuring examples of the data that can be generated and, importantly, how the data can be analysed. Furthermore, there are sections detailing tips, tricks and pitfalls to avoid, all written and peer-reviewed by leading experts in the field, making this an essential research companion.
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