METHODS ChemicalsAmpholines were obtained from LKB.Several different batch numbers were used during the course of this work.The quality of the gels varied only slightly, but precise reproduction of a separation should not be expected when the Ampholines are changed.Nonidet P-40 (HP-40) was purchased from Imperial Shell.SDS, manufactured by British Drug House Chemical Ltd., was purchased from Gallard-Schlesinger.Acrylamide, N,N'-methylenebisacrvlamide and N.N.N'.N'-tetramethvlethvlenediamine (TEMED)were purchased'frdm Eastman-Kodak.

High resolution two-dimensional electrophoresis of proteins.