The regulated release of peptide hormones depends on their packaging into dense-core vesicles (DCVs). Two models have been proposed for DCV cargo sorting. The “sorting by entry” model proposes that DCV cargos selectively enter nascent DCVs at the trans-Golgi network (TGN). The “sorting by exit” model proposes that sorting occurs by the post-TGN removal of non-DCV cargos and retention of mature DCV cargos. Here we show that the coiled-coil protein CCDC186 controls sorting by exit. Ccdc186 KO insulinoma cells secrete less insulin, fail to retain insulin and carboxypeptidase E in mature DCVs at the cell periphery, and fail to remove carboxypeptidase D from immature DCVs. A mutation affecting the endosome-associated recycling protein (EARP) complex causes similar defects in DCV cargo retention and removal. CCDC186 and EARP may act together to control the post-Golgi retention of cargos in mature DCVs.*   DCV    :   dense-core vesicle    TGN    :   trans-Golgi network    EARP    :   endosome-associated recycling protein    iDCV    :   immature dense-core vesicle    CPE    :   carboxypeptidase E    PC1/3    :   proprotein convertase 1/3    CgA    :   chromogranin A    MAO    :   monoamine oxidase    CPD    :   carboxypeptidase D    CD-MPR    :   cation-dependent mannose 6-phosphate receptor

CCDC186 controls dense-core vesicle cargo sorting by exit