ABSTRACT Protein clearance is fundamental to proteome health. In eukaryotes, it is carried by two highly conserved proteolytic systems, the ubiquitin-proteasome system (UPS) and the autophagy-lysosome pathway (ALP). Despite their pivotal role, the basal organization of the human protein clearance systems across tissues and cell types remains uncharacterized. Here, we interrogated this organization using diverse omics datasets. Relative to other protein-coding genes, UPS and ALP genes were more widely expressed, encoded more housekeeping proteins, and were more essential for growth, in accordance with their fundamental roles. Most of the UPS and ALP genes were nevertheless differentially expressed across tissues, and their tissue-specific upregulation was associated with tissue-specific functions, phenotypes, and disease susceptibility. The small subset of UPS and ALP genes that was stably expressed across tissues was more highly and widely expressed and more essential for growth than other UPS and ALP genes, suggesting that it acts as a core. Lastly, we compared protein clearance to other branches of the proteostasis network. Protein clearance and folding were closely coordinated across tissues, yet both were less pivotal than protein synthesis. Taken together, we propose that the proteostasis network is organized hierarchically and is tailored to the proteome needs. This organization could contribute to and illuminate tissue-selective phenotypes.

The landscape of cellular clearance systems across human tissues and cell types is shaped by tissue-specific proteome needs