Abstract Physiological homeostasis becomes compromised during aging, as a result of impairment of cellular processes, including transcription and RNA splicing. However, the molecular mechanisms leading to the loss of transcriptional fidelity are so far elusive, as are ways of preventing it. Here, we profiled and analyzed genome-wide, aging-related changes in transcriptional processes across different organisms: nematode worms, fruit flies, mice, rats and humans. The average transcriptional elongation speed (Pol-II speed) increased with age in all five species. Along with these changes in elongation speed we observed changes in splicing, including a reduction of unspliced transcripts and the formation of more circular RNAs. Two lifespan-extending interventions, dietary restriction and lowered insulin/Igf signaling, both reversed most of these aging-related changes. Genetic variants in Pol-II that reduced its speed in worms and flies increased their lifespan. Similarly, reducing Pol-II speed by overexpressing histone components, to counter age-associated changes in nucleosome positioning, also extended lifespan in flies and the division potential of human cells. Our findings uncover fundamental molecular mechanisms underlying animal aging and lifespan-extending interventions, and point to possible preventative measures.

Aging-associated changes in transcriptional elongation influence metazoan longevity