Abstract

The balance between selfing and outcrossing is a life history trait of major concern with deep evolutionary consequences in mixed mating species. Yet, our current understanding of the proximate and ultimate determinants of species mating system is still unsatisfactory and largely theoretical. Indeed, evolutionary biologists are still puzzled by the often dramatic variation of mating strategies within single species. Of particular concern is the extent to which environmental conditions shape patterns of variation and covariation of mating system components within species. Here, we address this concern in the common morning glory (Ipomoea purpurea) by taking advantage of an extensive dataset of floral traits, genetic estimates of selfing and inbreeding, and relevant environmental factors compiled for 22 populations of this species distributed along a disparate set of environments along Southeast and Midwest USA. Combining a powerful array of parametric and model-free statistical approaches, we robustly identify a set of natural and anthropogenic environmental factors underlying population-level variation in selfing, inbreeding, and flower morphology. Remarkably, individual mating system components are found to be associated with different environmental factors and only loosely associated with each other, and thus potentially under multiple different selective pressures. These results not only corroborate theoretical expectations of the significant role the environment plays in the local determination of mating systems, but also provide compelling evidence of complex underlying interactions between multiple evolutionary processes.