Abstract

A Gram-positive, motile, short-rod-shaped strain, designated YIM 004 T , was isolated from a forest-soil sample collected from Lijiang, Yunnan Province, China, and was investigated using a polyphasic taxonomic approach. The isolate contained chemotaxonomic markers that corresponded to those of its phylogenetic neighbour, Georgenia muralis , i.e. it possessed peptidoglycan type A4 α with lysine as the diagnostic cell-wall diamino acid, the predominant menaquinone was MK-8(H 4 ) and the major fatty acid was ai-C 15 : 0 . The G + C content of the genomic DNA was 72.9 mol%. Strain YIM 004 T exhibited a 16S rRNA gene sequence similarity of 97.3 % and a DNA–DNA relatedness value of 18 % with respect to G. muralis DSM 14418 T . On the basis of the phenotypic and genotypic differences between the isolate and G. muralis , strain YIM 004 T represents a novel species of the genus Georgenia , for which the name Georgenia ruanii sp. nov. is proposed. The type strain is YIM 004 T (=CCTCC AB 204065 T =DSM 17458 T =KCTC 19029 T ). In addition, an emended description of the genus Georgenia is presented .