Hepatocyte function and extracellular matrix geometry: long‐term culture in a sandwich configuration

Abstract

The FASEB JournalVolume 3, Issue 2 p. 174-177 Research Communications Hepatocyte function and extracellular matrix geometry: long-term culture in a sandwich configuration James C. Y. Dunn, James C. Y. Dunn Department of Surgery, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, 02114 USASearch for more papers by this authorMartin L. Yarmush, Martin L. Yarmush Department of Surgery, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, 02114 USA Department of Chemical and Biochemical Engineering, Rutgers University, Piscataway, New Jersey, 08854 USASearch for more papers by this authorHans G. Koebe, Hans G. Koebe Department of Surgery, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, 02114 USASearch for more papers by this authorRonald G. Tompkins, Corresponding Author Ronald G. Tompkins n/a@.dne Department of Surgery, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, 02114 USASend correspondence to: Ronald G. Tompkins, M.D., ScD., ACC 3A, Suite 364, Massachusetts General Hospital, Boston, MA 02114, USA.Search for more papers by this author James C. Y. Dunn, James C. Y. Dunn Department of Surgery, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, 02114 USASearch for more papers by this authorMartin L. Yarmush, Martin L. Yarmush Department of Surgery, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, 02114 USA Department of Chemical and Biochemical Engineering, Rutgers University, Piscataway, New Jersey, 08854 USASearch for more papers by this authorHans G. Koebe, Hans G. Koebe Department of Surgery, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, 02114 USASearch for more papers by this authorRonald G. Tompkins, Corresponding Author Ronald G. Tompkins n/a@.dne Department of Surgery, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, 02114 USASend correspondence to: Ronald G. Tompkins, M.D., ScD., ACC 3A, Suite 364, Massachusetts General Hospital, Boston, MA 02114, USA.Search for more papers by this author First published: 01 February 1989 https://doi.org/10.1096/fasebj.3.2.2914628Citations: 587AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinked InRedditWechat Abstract Adult rat hepatocytes cultured in a collagen sandwich system maintained normal morphology and a physiological rate of albumin secretion for at least 42 days. Hepatocytes cultured on a single layer of collagen gel essentially ceased albumin secretion within 1 wk but could recover function with the overlay of a second layer of collagen gel. This culture configuration more closely mimics the hepatocytes' in vivo environment and provides a simple method for their long-term maintenance.— Dunn, J. C. Y.; Yarmush, M. L.; Koebe, H. G.; Tompkins, R. G. Hepatocyte function and extracellular matrix geometry: long-term culture in a sandwich configuration. FASEB J. 3: 174-177; 1989. Citing Literature Volume3, Issue2February 1989Pages 174-177 RelatedInformation