Abstract

Angewandte Chemie International EditionVolume 50, Issue 31 p. 7008-7012 Communication The Discovery of 5-Formylcytosine in Embryonic Stem Cell DNA† M. Sc. Toni Pfaffeneder, M. Sc. Toni Pfaffeneder CIPSM, Fakultät für Chemie und Pharmazie, Ludwig-Maximilians-Universität München, Butenandtstrasse 5–13, 81377 München (Germany) http://www.carellgroup.de These authors contributed equally to this work.Search for more papers by this authorM. Sc. Benjamin Hackner, M. Sc. Benjamin Hackner CIPSM, Fakultät für Chemie und Pharmazie, Ludwig-Maximilians-Universität München, Butenandtstrasse 5–13, 81377 München (Germany) http://www.carellgroup.de These authors contributed equally to this work.Search for more papers by this authorDr. Matthias Truß, Corresponding Author Dr. Matthias Truß [email protected] Charité Universitätsklinikum, Otto-Heubner-Centrum für Kinder- und Jugendmedizin, Klinik für Allgemeine Pädiatrie, Labor für Pädiatrische Molekularbiologie, Ziegelstrasse 5–9, 10098 Berlin (Germany) These authors contributed equally to this work. Matthias Truß, Charité Universitätsklinikum, Otto-Heubner-Centrum für Kinder- und Jugendmedizin, Klinik für Allgemeine Pädiatrie, Labor für Pädiatrische Molekularbiologie, Ziegelstrasse 5–9, 10098 Berlin (Germany) Thomas Carell, CIPSM, Fakultät für Chemie und Pharmazie, Ludwig-Maximilians-Universität München, Butenandtstrasse 5–13, 81377 München (Germany) http://www.carellgroup.deSearch for more papers by this authorDipl.-Chem. Martin Münzel, Dipl.-Chem. Martin Münzel CIPSM, Fakultät für Chemie und Pharmazie, Ludwig-Maximilians-Universität München, Butenandtstrasse 5–13, 81377 München (Germany) http://www.carellgroup.deSearch for more papers by this authorDr. Markus Müller, Dr. Markus Müller CIPSM, Fakultät für Chemie und Pharmazie, Ludwig-Maximilians-Universität München, Butenandtstrasse 5–13, 81377 München (Germany) http://www.carellgroup.deSearch for more papers by this authorDipl.-Biochem. Christian A. Deiml, Dipl.-Biochem. Christian A. Deiml CIPSM, Fakultät für Chemie und Pharmazie, Ludwig-Maximilians-Universität München, Butenandtstrasse 5–13, 81377 München (Germany) http://www.carellgroup.deSearch for more papers by this authorProf. Dr. Christian Hagemeier, Prof. Dr. Christian Hagemeier Charité Universitätsklinikum, Otto-Heubner-Centrum für Kinder- und Jugendmedizin, Klinik für Allgemeine Pädiatrie, Labor für Pädiatrische Molekularbiologie, Ziegelstrasse 5–9, 10098 Berlin (Germany)Search for more papers by this authorProf. Dr. Thomas Carell, Corresponding Author Prof. Dr. Thomas Carell [email protected] CIPSM, Fakultät für Chemie und Pharmazie, Ludwig-Maximilians-Universität München, Butenandtstrasse 5–13, 81377 München (Germany) http://www.carellgroup.de Matthias Truß, Charité Universitätsklinikum, Otto-Heubner-Centrum für Kinder- und Jugendmedizin, Klinik für Allgemeine Pädiatrie, Labor für Pädiatrische Molekularbiologie, Ziegelstrasse 5–9, 10098 Berlin (Germany) Thomas Carell, CIPSM, Fakultät für Chemie und Pharmazie, Ludwig-Maximilians-Universität München, Butenandtstrasse 5–13, 81377 München (Germany) http://www.carellgroup.deSearch for more papers by this author M. Sc. Toni Pfaffeneder, M. Sc. Toni Pfaffeneder CIPSM, Fakultät für Chemie und Pharmazie, Ludwig-Maximilians-Universität München, Butenandtstrasse 5–13, 81377 München (Germany) http://www.carellgroup.de These authors contributed equally to this work.Search for more papers by this authorM. Sc. Benjamin Hackner, M. Sc. Benjamin Hackner CIPSM, Fakultät für Chemie und Pharmazie, Ludwig-Maximilians-Universität München, Butenandtstrasse 5–13, 81377 München (Germany) http://www.carellgroup.de These authors contributed equally to this work.Search for more papers by this authorDr. Matthias Truß, Corresponding Author Dr. Matthias Truß [email protected] Charité Universitätsklinikum, Otto-Heubner-Centrum für Kinder- und Jugendmedizin, Klinik für Allgemeine Pädiatrie, Labor für Pädiatrische Molekularbiologie, Ziegelstrasse 5–9, 10098 Berlin (Germany) These authors contributed equally to this work. Matthias Truß, Charité Universitätsklinikum, Otto-Heubner-Centrum für Kinder- und Jugendmedizin, Klinik für Allgemeine Pädiatrie, Labor für Pädiatrische Molekularbiologie, Ziegelstrasse 5–9, 10098 Berlin (Germany) Thomas Carell, CIPSM, Fakultät für Chemie und Pharmazie, Ludwig-Maximilians-Universität München, Butenandtstrasse 5–13, 81377 München (Germany) http://www.carellgroup.deSearch for more papers by this authorDipl.-Chem. Martin Münzel, Dipl.-Chem. Martin Münzel CIPSM, Fakultät für Chemie und Pharmazie, Ludwig-Maximilians-Universität München, Butenandtstrasse 5–13, 81377 München (Germany) http://www.carellgroup.deSearch for more papers by this authorDr. Markus Müller, Dr. Markus Müller CIPSM, Fakultät für Chemie und Pharmazie, Ludwig-Maximilians-Universität München, Butenandtstrasse 5–13, 81377 München (Germany) http://www.carellgroup.deSearch for more papers by this authorDipl.-Biochem. Christian A. Deiml, Dipl.-Biochem. Christian A. Deiml CIPSM, Fakultät für Chemie und Pharmazie, Ludwig-Maximilians-Universität München, Butenandtstrasse 5–13, 81377 München (Germany) http://www.carellgroup.deSearch for more papers by this authorProf. Dr. Christian Hagemeier, Prof. Dr. Christian Hagemeier Charité Universitätsklinikum, Otto-Heubner-Centrum für Kinder- und Jugendmedizin, Klinik für Allgemeine Pädiatrie, Labor für Pädiatrische Molekularbiologie, Ziegelstrasse 5–9, 10098 Berlin (Germany)Search for more papers by this authorProf. Dr. Thomas Carell, Corresponding Author Prof. Dr. Thomas Carell [email protected] CIPSM, Fakultät für Chemie und Pharmazie, Ludwig-Maximilians-Universität München, Butenandtstrasse 5–13, 81377 München (Germany) http://www.carellgroup.de Matthias Truß, Charité Universitätsklinikum, Otto-Heubner-Centrum für Kinder- und Jugendmedizin, Klinik für Allgemeine Pädiatrie, Labor für Pädiatrische Molekularbiologie, Ziegelstrasse 5–9, 10098 Berlin (Germany) Thomas Carell, CIPSM, Fakultät für Chemie und Pharmazie, Ludwig-Maximilians-Universität München, Butenandtstrasse 5–13, 81377 München (Germany) http://www.carellgroup.deSearch for more papers by this author First published: 30 June 2011 https://doi.org/10.1002/anie.201103899Citations: 414 † We thank the Deutsche Forschungsgemeinschaft (DFG) for financial support through the SFB program (SFB 749, 646, and TRR54) and by grant CA275 8/4. Further support was obtained from the Excellence Cluster (Center for Integrative Protein Science, CiPSM) and NGFNplus (01GS0870). Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onEmailFacebookTwitterLinkedInRedditWechat Graphical Abstract Touching base: Sophisticated mass spectrometry has shown that 5-formylcytosine is a constituent of mammalian embryonic stem cell DNA. This base is likely produced from methylcytosine via hydroxymethylcytosine (see scheme), and it may serve as an intermediate in the long searched for pathway of active DNA demethylation. Supporting Information Detailed facts of importance to specialist readers are published as ”Supporting Information”. Such documents are peer-reviewed, but not copy-edited or typeset. They are made available as submitted by the authors. Filename Description anie_201103899_sm_miscellaneous_information.pdf916.1 KB miscellaneous_information Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article. References 1 1aR. Bonasio, S. J. Tu, D. Reinberg, Science 2010, 330, 612–616; 10.1126/science.1191078 CASPubMedWeb of Science®Google Scholar 1bP. A. Jones, D. 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