The human superior temporal plane, the site of the auditory cortex, displays a high inter-individual macro-anatomical variation. This questions the validity of curvature based alignment (CBA) methods for in vivo imaging data. Here, we have addressed this issue by developing CBA+, which is a cortical surface registration method that uses prior macro-anatomical knowledge. We validate this method by using cyto-architectonic areas on ten individual brains (which we make publicly available). Compared to volumetric and standard surface registration, CBA+ results in a more accurate cyto-architectonic auditory atlas. The improved correspondence of micro-anatomy following the improved alignment of macro-anatomy validates the superiority of CBA+ compared to CBA. In addition, we use CBA+ to align in vivo and post mortem data. This allows projection of functional and anatomical information collected in vivo onto the cyto-architectonic areas, which has potential to contribute to ongoing debate on the parcellation of the human auditory cortex.

Improving a probabilistic cytoarchitectonic atlas of auditory cortex using a novel method for inter-individual alignment