Abstract

Research Article1 October 1989free access The argininosuccinate lyase gene of Chlamydomonas reinhardtii: an important tool for nuclear transformation and for correlating the genetic and molecular maps of the ARG7 locus. R. Debuchy R. Debuchy Department of Molecular Biology, University of Geneva, Switzerland. Search for more papers by this author S. Purton S. Purton Department of Molecular Biology, University of Geneva, Switzerland. Search for more papers by this author J.D. Rochaix J.D. Rochaix Department of Molecular Biology, University of Geneva, Switzerland. Search for more papers by this author R. Debuchy R. Debuchy Department of Molecular Biology, University of Geneva, Switzerland. Search for more papers by this author S. Purton S. Purton Department of Molecular Biology, University of Geneva, Switzerland. Search for more papers by this author J.D. Rochaix J.D. Rochaix Department of Molecular Biology, University of Geneva, Switzerland. Search for more papers by this author Author Information R. Debuchy1, S. Purton1 and J.D. Rochaix1 1Department of Molecular Biology, University of Geneva, Switzerland. The EMBO Journal (1989)8:2803-2809https://doi.org/10.1002/j.1460-2075.1989.tb08426.x PDFDownload PDF of article text and main figures. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info The argininosuccinate lyase (ASL) gene of Chlamydomonas reinhardtii has been cloned using four oligonucleotide probes corresponding to highly conserved regions of the ASL polypeptide sequence. The identity of the gene was confirmed by partial sequencing. It is unique, contains several introns and spans a region less than 7.8 kb that includes highly repetitive sequences. Using a particle gun, a reliable nuclear transformation system has been established by complementing three mutants deficient in ASL activity with the wild-type ASL gene. Analysis of the transformants reveals variable patterns of integration of the transforming DNA into the nuclear genome. Previous work has mapped the mutations in the mutants arg2 and arg7 to either end of the ARG7 locus 1.0 to 1.6 recombination map units apart. Our transformation results show that these two mutations are located within a region of 7.8 kb. This allows for the first correlation of the recombination map and the molecular map at the ARG7 locus and indicates a high recombination frequency in this region of the nuclear genome. Previous ArticleNext Article Volume 8Issue 101 October 1989In this issue RelatedDetailsLoading ...