The FASEB JournalVolume 23, Issue 10 p. 3393-3404 Research CommunicationFree to Read Genetic requirements for Staphylococcus aureus abscess formation and persistence in host tissues Retraction(s) for this article Erratum Volume 24Issue 2The FASEB Journal pages: 648-648 First Published online: February 1, 2010 Alice G. Cheng, Alice G. Cheng Department of Microbiology, University of Chicago, Chicago, Illinois, USASearch for more papers by this authorHwan Keun Kim, Hwan Keun Kim Department of Microbiology, University of Chicago, Chicago, Illinois, USASearch for more papers by this authorMonica L. Burts, Monica L. Burts Department of Microbiology, University of Chicago, Chicago, Illinois, USASearch for more papers by this authorThomas Krausz, Thomas Krausz Department of Pathology, University of Chicago, Chicago, Illinois, USASearch for more papers by this authorOlaf Schneewind, Corresponding Author Olaf Schneewind [email protected] Department of Microbiology, University of Chicago, Chicago, Illinois, USA Department of Pathology, University of Chicago, Chicago, Illinois, USACorrespondence: Department of Microbiology, University of Chicago, 920 East 58th St., Chicago, IL 60637, USA. E-mail: [email protected]Search for more papers by this authorDominique M. Missiakas, Dominique M. Missiakas Department of Microbiology, University of Chicago, Chicago, Illinois, USASearch for more papers by this author Alice G. Cheng, Alice G. Cheng Department of Microbiology, University of Chicago, Chicago, Illinois, USASearch for more papers by this authorHwan Keun Kim, Hwan Keun Kim Department of Microbiology, University of Chicago, Chicago, Illinois, USASearch for more papers by this authorMonica L. Burts, Monica L. Burts Department of Microbiology, University of Chicago, Chicago, Illinois, USASearch for more papers by this authorThomas Krausz, Thomas Krausz Department of Pathology, University of Chicago, Chicago, Illinois, USASearch for more papers by this authorOlaf Schneewind, Corresponding Author Olaf Schneewind [email protected] Department of Microbiology, University of Chicago, Chicago, Illinois, USA Department of Pathology, University of Chicago, Chicago, Illinois, USACorrespondence: Department of Microbiology, University of Chicago, 920 East 58th St., Chicago, IL 60637, USA. E-mail: [email protected]Search for more papers by this authorDominique M. Missiakas, Dominique M. Missiakas Department of Microbiology, University of Chicago, Chicago, Illinois, USASearch for more papers by this author First published: 12 June 2009 https://doi.org/10.1096/fj.09-135467Citations: 34Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL ABSTRACT Staphylococcus aureus infections are associated with abscess formation and bacterial persistence; however, the genes that enable this lifestyle are not known. We show here that following intravenous infection of mice, S. aureus disseminates rapidly into organ tissues and elicits abscess lesions that develop over weeks but cannot be cleared by the host. Staphylococci grow as communities at the center of abscess lesions and are enclosed by pseudocapsules, separating the pathogen from immune cells. By testing insertional variants in genes for cell wall-anchored surface proteins, we are able to infer the stage at which these molecules function. Fibrinogen-binding proteins ClfA and ClfB are required during the early phase of staphylococcal dissemination. The heme scavenging factors IsdA and IsdB, as well as SdrD and protein A, are necessary for abscess formation. Envelope-associated proteins, Emp and Eap, are either required for abscess formation or contribute to persistence. Fluorescence microscopy revealed Eap deposition within the pseudocapsule, whereas Emp was localized within staphylococcal abscess communities. Antibodies directed against envelope-associated proteins generated vaccine protection against staphylococcal abscess formation. Thus, staphylococci employ envelope proteins at discrete stages of a developmental program that enables abscess formation and bacterial persistence in host tissues.—Cheng, A. G., Kim, H. K., Burts, M. L., Krausz, T., Schneewind, O., Missiakas, D. M. Genetic requirements for Staphylococcus aureus abscess formation and persistence in host tissues. FASEB J. 23, 3393–3404 (2009). www.fasebj.org Citing Literature Volume23, Issue10October 2009Pages 3393-3404 RelatedInformation