Over the past 10 years, interest in plant biostimulants (PBs) has been on the rise compelled by the growing interest of scientists, extension specialists, private industry, and growers in integrating these products in the array of environmentally friendly tools that secure improved crop performance and yield stability. Based on the new EU regulation PBs are defined through claimed agronomic effects, such as improvement of nutrient use efficiency, tolerance to abiotic stressors and crop quality. This definition entails diverse organic and inorganic substances and/or microorganisms such as humic acids, protein hydrolysates, seaweed extracts, mycorrhizal fungi, and N-fixing bacteria. The current mini-review provides an overview of the direct (stimulatory on C and N metabolism) and indirect (enhancing nutrient uptake and modulating root morphology) mechanisms by which microbial and non-microbial PBs improve nutrient efficiency, plant performance, and physiological status, resilience to environmental stressors and stimulate plant microbiomes. The scientific advances underlying synergistic and additive effects of microbial and non-microbial PBs are compiled and discussed for the first time. The review identifies several perspectives for future research between the scientific community and private industry to design and develop a second generation of PBs products (biostimulant 2.0) with specific biostimulatory action to render agriculture more sustainable and resilient.

Synergistic Biostimulatory Action: Designing the Next Generation of Plant Biostimulants for Sustainable Agriculture