The efficient and accurate separation of immunoglobulin G (IgG) plays a vital role for disease diagnosis and therapy, but it is always hampered by the huge geometric size and complex structure of IgG. In this work, an amorphous Fe/Co bimetallic organic framework (denoted as PMOF-Fe/Co) is fabricated for IgG separation, with octa-carboxyl polyhedral oligomeric silsesquioxane (OCPOSS) as modulator for the first time. Benefiting from the rigid nanostructure and competitive coordination of OCPOSS, the aperture of PMOF-Fe/Co is enlarged to ∼20 nm along with the generation of enormous structural defects, which enables the accommodation of protein species with high molecular weights and large sizes. OCPOSS is also found exerting a positive impact on mediating the specific recognition and adsorption ability of PMOF-Fe/Co towards IgG through metal affinity, hydrophilic and hydrophobic interactions. Consequently, the multimode and multivalent affinity of PMOF-Fe/Co gives rise to an extraordinary adsorption capacity (2691.7 mg g

Polyhedral oligomeric silsesquioxane-modulated mesoporous amorphous bimetallic organic frameworks for the efficient isolation of immunoglobulin G