Significance This study reports the cloning of a tomato gene, SlKLUH , that controls fruit mass by increased cell layers and delayed fruit ripening. In addition, we identified a potential regulatory SNP in the promoter of SlKLUH that is significantly associated with the fruit mass. Altogether, our study encompasses several genetic analyses, as well as association mapping, plant transformation experiments, and phenotypic evaluations to offer insights into the molecular basis of the regulation of tomato fruit mass, a critical trait in the domestication of fruit and vegetable crops.
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