Abstract Protein S‐sulfhydration (forming ‐S‐SH adducts from cysteine residues) is a newly defined oxidative posttranslational modification and plays an important role in H 2 S‐mediated signaling pathways. In this study we report the first selective, “tag‐switch” method which can directly label protein S‐sulfhydrated residues by forming stable thioether conjugates. Furthermore we demonstrate that H 2 S alone cannot lead to S‐sulfhydration and that the two possible physiological mechanisms include reaction with protein sulfenic acids (P‐SOH) or the involvement of metal centers which would facilitate the oxidation of H 2 S to HS . .

Detection of Protein S‐Sulfhydration by a Tag‐Switch Technique

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