ResearchHub Logo
Currency
Display format
RSCUSD

Reducing resistance allele formation in CRISPR gene drive

Authors
Jackson ChamperJingxian LiuPhilipp Messer
Journal
Proceedings of the National Academy of Sciences
Published
May 7, 2018

Abstract

Significance A functioning gene drive mechanism could fundamentally change our strategies for the control of vector-borne diseases, such as malaria, dengue, and Zika. CRISPR homing gene drive promises such a mechanism, which could be used to rapidly spread genetic modifications among the mosquitoes that transmit these diseases. However, recent studies have shown that current drives would likely be unable to spread in insect populations due to the high rate at which resistance will evolve. In this study, we provide an experimental demonstration that guide RNA multiplexing can successfully reduce resistance rates but also find that such an approach would still need to be combined with additional strategies to create drives that are efficient enough for use in wild populations.

Supporters

Support the authors with ResearchCoin

Topics

DOI

10.1073/pnas.1720354115

License

Unknown License
Reducing resistance allele formation in CRISPR gene drive | ResearchHub