The scattering characteristics of particles with different transparency are investigated using interferometric particle imaging. Based on optical transfer matrix theory, the interference defocusing images formed by different number of emitting points are simulated. We found that the speckle frequency of the defocusing image will increase when the number of the emitting points increases. The microscopic and interference images of polystyrene, cell and sand particles are obtained and proved that the lower the transparency of the particle, the higher the speckle frequency of the defocusing image. The experimental results showed agreement with the simulation results. This conclusion can be used to judge the transparency of particles and to distinguish particles in mixed particle field.