Abstract

We demonstrate longitudinal drug response imaging of tumor spheroids by integrating a spheroid cultivation chamber and the dynamic optical coherence tomography (DOCT) microscope. The cultivation chamber supports the spheroids with 5% of CO2 and a temperature of 37°C. In contrast to our previous cross-sectional time-course imaging method, this newly integrated system enables longitudinal time-course imaging of a single sample, and hence enables high-time-resolution imaging of a living sample. It successfully revealed the fine temporal and spatial responses of human breast cancer (MCF-7 cell-line) spheroids to paclitaxel (PTX) and doxorubicin hydrochloride (DOX).