Abstract

This research prepared gelatinized waxy maize starch (WMS), low-amylose maize starch (LAS), and high-amylose maize starch (HAS) with different glutathione (GSH) content (5, 10, and 15 %) using high hydrostatic pressure (HHP) at 600 MPa. Scanning electron microscopy (SEM) revealed damaged morphology of WMS and complete swelled granules of LAS and HAS with different degree of gelatinization (DG) values, 92.86, 59.36, and 17.45 %, respectively. Fourier transform infrared spectroscopy (IR spectra), laser confocal micro-Raman (LCM-Raman) spectroscopy, and X-ray diffraction (XRD) results suggested that the crystallinity content of gelatinized WMS and HAS with addition of GSH was higher than that of LAS, and the gelatinized LAS and HAS were mainly of C type and V type, respectively. The resistant starch of LAS (25.15 %) and HAS (34.76 %) increased with GSH addition. The crosslinking between GSH and amylose/amylopectin caused changes in physicochemical properties. This study will provided theoretical basis for GSH usage in food industry.