Scientist with 14 years lab experience including 3 years in the pharmaceutical industry. Expertise in drug development, mass spectrometry, pharmacokinetics/pharmacodynamics, molecular cloning and recombinant protein production.

University of California--San Diego

Biochemistry and Cell Biology BASc '14, University of California--San Diego

University of California--Davis

Pharmacology & Toxicology PhD '24, University of California--Davis

Pharmacology | Cancer Research | Art | Music

Pharmacology

Molecular Biology

Artificial Intelligence

Safety Research

Cancer Research

Pulmonary And Respiratory Medicine

Applied Psychology

Biomaterials

Cognitive Neuroscience

Cellular And Molecular Neuroscience

Oncology

Biochemistry

Biological Psychiatry

Clinical Psychology

Rheumatology

Finance

Physiology

Organic Chemistry

Dermatology

Spectroscopy

Materials Chemistry

Immunology

Pharmaceutical Science

Surgery

Pathology And Forensic Medicine

Focus areas: cell biology, stem cell biology, cardiac biology,  cell/tissue/organ engineering, organoids, in vitro models, drug discovery, cell reprogramming, cell and gene therapy, biotechnology, biomaterials, microfluidics & microtechnologies, longevity biotechnology, experimental design.

Open & decentralized science | Lab automation | Bioengineering

Biology

Dr. Nelson is an Associate Professor with tenure in the Department of Biochemistry, Biophysics, and Molecular Biology at Iowa State University.  His lab investigates the molecular mechanisms of DNA replication and repair with a focus on infectious organisms.

Enzymology of DNA Replication and Repair

Experimental Methods

Nick Oldberg's Notebook

Gel Loading Calculator for Western Blotting

This is something I made which has been a huge help when running western blots. It will take samples at any concentration and normalize them all to each other, making prep for loading your gel a breeze. The beauty of this is you can easily mess with any parameter you'd like without having to redo any math. Just edit the red parts and the green shaded columns are your aliquot volumes. File Link: https://docs.google.com/spreadsheets/d/1DxwFme3zP4Jw8HLSPWuod7Rc72npsZvv/edit?usp=sharing&ouid=111441827851825405038&rtpof=true&sd=true Here's the calculator:  I'll take you through how to use it, step by step: Step 1: Run BCA or other protein quantitation assay on your lysates A protocol for running the BCA itself can be found here: https://www.researchhub.com/post/2000/bca-protein-quantitation-assayAny protein quantitation assay can be used, I personally prefer BCA Step 2: Input Your ValuesColumns 1 and 2: Fill with sample descriptions of your choosing, such as cell line, treatment, etc. Whatever you'd like to put in to identify each sample, you have 2 columns to do it.Column 3: Input your protein concentration values for each sample here in ug/uL.  Step 3: Input Values into Calculator Bottom SectionThese values are specific to the type of gel you will be running, I will go through these one by one:Reducing Agent Dilution Factor I use 1M DTT as my reducing reagent, with 50mM final concentration in my gel-ready samples. This is a 20x dilution, therefore I filled in a 20 here. This may be different depending on what you're using. If it's 10x reducing agent, then put a 10. etc. Laemmle Buffer Dilution FactorMy lab uses 4x Laemmle buffer stock diluted to 1x final concentration in my gel-ready samples. Therefore, this is a 4x dilution, so I filled in a 4 here. As above, this may differ depending what your lab uses. If you're using 6x, put a 6 here. etc. Volume (uL) Loaded per Well of Gel:  This volume changes depending on well size and gel type, so fill in the volume you're planning to load per well.#/Times Samples Will be Run:Enter how many times you will be running each sample. If you're running a sample 3 times, put a 3 here. This calculator has a built-in 10% surplus so you will have enough volume for all 3 runs + a bit extra. Additional Dilution FactorWhen a “1” is in this cell the “Protein Loaded per Well (ug)” will be the highest it can be, given your sample concentrations. This means your lowest concentration sample will have 0 uL lysis buffer and be comprised entirely of reducing agent, Laemmle buffer, and your lysate sample. If the protein loaded per well (ug) is too high, tinker with this number until the amount loaded per well is what you'd like. Step 4: Prep Your SamplesWe're done inputting numbers! Proceed to the green shaded part of the chart and start prepping your samples! All samples will end up equal in volume and protein concentration.

Gel Loading Calculator for Western Blotting

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