Plant Growth - Latin Jazz - Summary

This was the Latin Jazz treatment (Various Artists), the fourth experimental treatment with a sound running throughout the entire time course for the experiment "Effects of music on lettuce germination and growth in a hydroponic system" (1) My thoughts and comments are documented below.

This is a time-lapse from the front of the lettuce growing.

This is a time-lapse of the lettuce growing from above.

Run Specific Data:

Below are the growth graphs of the 6 pods over time.

Figure 1. Seedlings sprouted. The number of seedlings were observed sprouting from 5 seeds planted in each pod. The average and standard deviation of 6 biological replicates are shown. On day 6, true leaves started to emerge. Seedlings were pruned back to 1 seed per pod to ensure optimal growth.

a.

b.

c.

d.

Fig 2. Leaf growth characteristics. a-c. True leaf characteristics (not cotyledon) were counted/measured at each time point. The number (a), width (b), length (c), and area (d) were all documented. The average and standard deviation of 6 biological replicates are shown.

Figure 3. Plant wet weight at harvest. Plants were weighed and had the weight of the wet pod and basket subtracted from them to get the plant weight. The average and standard deviation of 6 biological replicates are shown.

Fig 4. Water consumption of plants. Water consumption was determined by measuring the water level in the aerogarden at each time point. The formula: water consumption (mL) = 325.5 (ml/cm) x change in water level (cm) was used. The formula was derived from measuring the change in water level and corresponding water height measurements (2).

a.

b.

c.

d.

Fig 5. Environmental parameters of experimental setup. a-b. environmental parameters of the air in the cabinet. The temperature (a) humidity (b) was recorded throughout the experiment. c-d. environmental parameters of the water in the aerogarden, inside the cabinet. The temperature (c) humidity (d) was recorded throughout the experiment.

Cumulative Data:

Below are the growth graphs of the 6 pods over time, when compared to the other runs (3,-6)

Figure 6. Seedlings sprouted. The number of seedlings were observed sprouting from 5 seeds planted in each pod. The average and standard deviation of 6 biological replicates are shown.

a.

b.

c.

d.

Fig 7. Leaf growth characteristics. a-c. True leaf characteristics (not cotyledon) were counted/measured at each time point. The number (a), width (b), length (c), and area (d) were all documented. The average and standard deviation of 6 biological replicates are shown.

Figure 8. Plant wet weight at harvest. Plants were weighed and had the weight of the wet pod and basket subtracted from them to get the plant weight. The average and standard deviation of 6 biological replicates are shown.

Fig 9. Water consumption of plants. Water consumption was determined by measuring the water level in the aerogarden at each time point. The formula: water consumption (mL) = 325.5 (ml/cm) x change in water level (cm) was used. The formula was derived from measuring the change in water level and corresponding water height measurements (2).

Thoughts from the experiment:

Overall, this run has some marked differences from the other sound treatments. I think this is chiefly because there was a heat wave at the time of the experiment. My apartment does not have a good ability to cool down. Thus, I think that the temperature was too hot for the lettuce to grow appropriately. When the lettuces were exposed continuously to the Latin Jazz, all parameters were lower. I think that this experiment needs to be redone because of this aberration in the control settings. I don't love the idea, but it does not make sense to include it in the final analysis.

This sound treatment resulted in less water being consumed than the control group, again because of the heat. The plants also weighed less at the end of the experiment. A sigmoidal curve fits the data better, and it is all above positive, which I am glad to see. I would love any insights that people would have.

The dB average for the Nature Sounds treatment was 60.44 +/- 5.24 dB. There seem to be frequencies ranging from 50Hz-3kHz across the Playlist (7). I think that there is a lot of variation in this playlist and would love to get some help analyzing the spectral peaks to make sense of this.

There was still a little bit of tip burn occurring by the end of the experiment, but it seems not as much as some of the other runs. So far the tip burn is just being noted.

There was not any data recorded on Day 2 of the experiment because I was out of town. There is not any water readings from days 4-10 because the water probe that I had broke.

I would love to hear the opinions of others and they would be greatly appreciated :)

Raw data can be found here

References

Delyea, Cole. Effects Of Music On Lettuce Germination And Growth In A Hydroponic System. Preregistration (V3). ResearchHub [Internet]. 2023 Nov 17 [cited 2024 Jun 15. Available from: https://www.researchhub.com/post/1164/effects-of-music-on-lettuce-germination-and-growth-in-a-hydroponic-system-preregistration-v3. doi:10.55277/ResearchHub.w177k7ic
Delyea, Cole. Volume Of Aerogarden Based On Water Height. ResearchHub [Internet]. 2024 Jan 6 [cited 2024 Jun 15]. Available from: https://www.researchhub.com/post/1437/volume-of-aerogarden-based-on-water-height. doi:10.55277/ResearchHub.yzb05fym
Delyea, Cole. Plant Growth - No Sound Control - Summary. ResearchHub [Internet]. 2024 Feb 5 [cited 2024 Jun 15]. Available from: https://www.researchhub.com/post/1569/plant-growth-no-sound-control-summary . doi:10.55277/ResearchHub.m884jc3u
Delyea, Cole. Plant Growth - Buddhist Mantra - Summary. ResearchHub [Internet]. 2024 Apr 9 [cited 2024 Jun 15]. Available from: https://www.researchhub.com/post/2011/plant-growth-buddhist-mantra-summary . doi:10.55277/ResearchHub.hf9jnzbi
Delyea, Cole. Plant Growth - Music for Plants - Summary. ResearchHub [Internet]. 2024 May 7 [cited 2024 Jun 15]. Available from: https://www.researchhub.com/post/2221/plant-growth-music-for-plants-summary . doi:10.55277/ResearchHub.ezgm7qzn
Delyea, Cole. Plant Growth - Nature Sounds - Summary. ResearchHub [Internet]. 2024 June 15 [cited 2024 Aug 5]. Available from: https://www.researchhub.com/post/2780/plant-growth-nature-sounds-summary. doi:10.55277/ResearchHub.eml6xr4a
Delyea, Cole. Latin Jazz Sound Characteristics (Various). ResearchHub [Internet]. 2024 Aug 4 [cited 2024 Jun 15]. Available from: https://www.researchhub.com/post/3031/latin-jazz-sound-characteristics-various-artists. doi:10.55277/ResearchHub.jo9s63ck

Plant Growth - Latin Jazz - Summary

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Plant Growth - Latin Jazz - Summary

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