Abstract Untargeted metabolomics is often used in studies that aim to trace the metabolic profile in a broad context, with the data-dependent acquisition (DDA) mode being the most commonly used method. However, this approach has the limitation that not all detected ions are fragmented in the data acquisition process, in addition to the lack of specificity regarding the process of fragmentation of biological signals. The present work aims to extend the detection of biological signals and contribute to overcoming the fragmentation limits of the DDA mode with a dynamic procedure that combines experimental and in silico approaches. Metabolomic analysis was performed on three different species of actinomycetes using liquid chromatography coupled to mass spectrometry. The data obtained were preprocessed by the MZmine software and processed by the custom package, RegFilter. RegFilter allowed the coverage of the entire chromatographic run and the selection of precursor ions for fragmentation that were previously missed in DDA mode. Most of the ions selected by the tool could be annotated through three levels of annotation, presenting biological relevant candidates. In addition, the tool offers the possibility of creating local spectral libraries curated according to the user’s interests. Thus, the adoption of a dynamic analysis flow using RegFilter allowed for detection optimization of biological signals, previously absent in the DDA mode. In addition, this workflow enables the creation and search of in-house tailored custom libraries.