Non-oriented electrical steel typically operates under mechanical stress when utilized as a motor core, leading to significant impacts on its magnetostrictive properties. Additionally, electrical steel containing different levels of Si content may demonstrate varying degrees of magnetostrictive properties. Therefore, this paper aims to test the magnetostrictive properties of four types of non-oriented electrical steel sheets, each with different Si contents and under various tensile stresses, by employing a monolithic magnetostrictive measurement system. The findings indicate a decline in the magnetostrictive strain of electrical steel as the Si content increases. Under the influence of tensile stress, the magnetostrictive effect of electrical steel decreases. This trend changes with the increase of Si content, showing an initial rise followed by a decline. It is worth noting that the magnetostrictive strain of 6.5% Si is minimally affected by both the increase in magnetic flux density and tensile stress. Finally, based on the micromagnetic mechanism and micro grain structure of the magnetostrictive effect of electrical steel, this paper describes the variation pattern of the magnetostrictive effect of electrical steel with varying Si content under the influence of tensile stress.