Soil organic matter has recently been implicated as an important sink for atmospheric carbon dioxide (CO 2 ). However, the relative impacts of various agricultural management practices on soil organic matter dynamics and, therefore, C sequestration at spatial scales larger than a single plot or times longer than the typical three year experiment have rarely been reported. Results of maintaining agricultural management practices in the forest-derived soils of the eastern Corn (Zea mays L.) Belt states of Kentucky, Michigan, Ohio and Pennsylvania (USA) were studied. We found annual organic C input and tillage intensity were the most important factors in affecting C sequestration. The impact of rotation on C sequestration was primarily related to the way it altered annual total C inputs. The removal of above-ground plant biomass and use of cover crops were of lesser importance. The most rapid changes in soil organic matter content occurred during the first five years after a management practice was imposed with slower changes occurring thereafter. Certain management practices, e.g. no-tillage (NT), increased the soil's ability to sequester atmospheric CO 2 . The impact of this sequestration will be significant only when these practices are used extensively on a large percentage of cropland and when the C-building practices are maintained. Any soil C sequestered will be rapidly mineralized to CO 2 if the soil organic matter building practices are not maintained.