Abstract The special properties of rare earth elements (REE) have effectively broadened their application fields. How to accurately recognize and efficiently separate target rare earth ions with similar radii and chemical properties remains a formidable challenge. Here, precise two‐dimensional (2D) heterogeneous channels are constructed using engineered E. coli membranes between graphene oxide (GO) layers. The difference in binding ability and corresponding conformational change between Lanmodulin (LanM) and rare earth ions in the heterogeneous channel allows for precisely recognizing and sieving of scandium ions (Sc 3+ ). The engineered E. coli membranes not only can protect the integrity of structure and functionality of LanM, the rich lipids and sugars, but also help the Escherichia coli ( E. coli ) membranes closely tile on the GO nanosheets through interaction, preventing swelling and controlling interlayer spacing accurately down to the sub‐nanometer. Apparently, the 2D heterogeneous channels showcase excellent selectivity for trivalent ions (SF Fe /Sc ≈3), especially for Sc 3+ ions in REE with high selectivity (SF Ce/Sc ≈167, SF La/Sc ≈103). The long‐term stability and tensile strain tests verify the membrane's outstanding stability. Thus, this simple, efficient, and cost‐effective work provides a suggestion for constructing 2D interlayer heterogeneous channels for precise sieving, and this valuable strategy is proposed for the efficient extraction of Sc.

Constructing Two‐Dimensional Heterostructure Channels with Engineered Biomembrane and Graphene for Precise Scandium Sieving