Healthy Research Rewards
ResearchHub is incentivizing healthy research behavior. At this time, first authors of open access papers are eligible for rewards. Visit the publications tab to view your eligible publications.
Got it
CT
Chee Tan
Author with expertise in Regulation of RNA Processing and Function
Achievements
Cited Author
Open Access Advocate
Key Stats
Upvotes received:
0
Publications:
2
(50% Open Access)
Cited by:
259
h-index:
6
/
i10-index:
5
Reputation
Biology
< 1%
Chemistry
< 1%
Economics
< 1%
Show more
How is this calculated?
Publications
10

Local Regulation and Function of Importin-β1 During Transcription-Dependent Plasticity

Yan Lee et al.Dec 3, 2020
SUMMARY Activity-dependent transcription is critical for the encoding of long-term memories. Regulated nuclear entry of soluble proteins is one method to relay synaptic signals to the nucleus to couple neuronal excitation with transcription. To date, the role of importin-β1 in nuclear shuttling of proteins during activity-dependent transcription has always been inferred but not directly investigated. In this study, we demonstrate activity-dependent nuclear accumulation of importin-β1 from the soma and the synapto-dendritic compartments. Importantly, inhibition of importin-β1 mediated nuclear import during synaptic stimulation impairs long-term plasticity. We show evidence that importin-β1 mRNA-ribosome complex is distributed throughout the synapto-dendritic compartment and synaptic stimulation induces importin-β1 local protein synthesis. Finally, we identified candidate proteins that associate with importin-β1 at the synapse and characterize NDRG1 as an importin-β1 interactor that undergoes activitydependent translocation into the nucleus. Collectively, our results highlight the crucial role of importin-β1 in nuclear import of proteins during long-term plasticity.
10
Citation3
0
Save